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br Materials and methods br Results Because of the
2020-08-21
Materials and methods Results Because of the specific binding of DGKε to an arachidonoyl group, there was a particular interest to evaluate the behavior of 2-AG with this isoform of DGK. The substrate specificity and kinetic constants for DGKε has been recently reported [13]. Using the preferr
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br Electron transfer pathway Intramolecular
2020-08-21
Electron transfer pathway Intramolecular (or inter-domain) electron transfer (IET) in CDH has been studied in much depth [], and similar IET occurs in CcPDH, from the PQQ cofactor in the AA12 domain to the heme b in the AA8 domain. When two kinds of electron acceptors are used for measuring enzym
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HvPap a member of the cathepsin F
2020-08-21
HvPap-1, a member of the cathepsin F-like family, was identified in germinating barley seeds (Sreenivasulu et al., 2008). This enzyme participates in the mobilization of stored proteins (mainly hordeins) during the last phase of grain germination, and its activity is modulated by a propeptide or/and
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Cytochromes P CYP are a superfamily of
2020-08-21
Cytochromes P450 (CYP 450) are a superfamily of hemoproteins containing a heme cofactor, which are widely distributed in animals, plants and microbes (Hannemann et al., 2007). CYP450 exhibits a variety of biological functions including biotransformation of drugs, detoxification of endogenous and xen
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The importance of mitochondria as site of aerobic
2020-08-21
The importance of mitochondria as site of aerobic oxidation of metabolic fuels, such as FA, has been recognized for a long time. It has been observed that the number of mitochondria may vary in response to cellular metabolic requirements and hormonal environment (Gao et al., 2014). In order to susta
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NPC L NPC Niemann Pick C
2020-08-21
NPC1L1 (NPC1 [Niemann-Pick C-like-1]-like intracellular cholesterol transporter) is the rate-limiting transmembrane transporter for cholesterol 2×Taq PCR Master Mix(with dye) from the intestinal lumen, and it mediates the absorption of both dietary and biliary cholesterol., , NPC1L1 is the target
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br STAR Methods br Acknowledgments We are
2020-08-21
STAR★Methods Acknowledgments We are grateful to Amaia Lujambio, James Fagin, and Ramon Parsons for providing cell lines and Evripidis Gavathiotis for critical reading of the manuscript. We would like to thank Saboor Hekmaty for providing expertise with RNA-seq analysis. P.I.P. would like to ac
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br Materials and methods br Statistical analysis
2020-08-20
Materials and methods Statistical analysis All statistical analyses were performed using SPSS software version 20. Protein ARCA Cy3 EGFP mRNA data of DNMTs and clinicopathological parameters were collected. Mann-Whitney U Test was used to assess the differential expression of nonparametric da
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To date little is known about
2020-08-20
To date, little is known about the regulation of PCD through phytocystatin inhibitory activity. Inhibitors that may regulate leaf senescence (Huang et al., 2001, Tajima et al., 2011) or may inhibit PCD induced by abiotic factors, such as mechanical tissue damage or oxidative stress caused by cold (B
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Furthermore software engineering practices are important for
2020-08-20
Furthermore, software engineering practices are important for addressing productivity problems in scientific software. Even though the speed of the hardware is rapidly increasing, the additional complexity makes it more difficult for scientists to be productive developers. According to Faulk et al.,
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Go 6976 The different diagnostic outcomes of the two assays
2020-08-20
The different diagnostic outcomes of the two assays are also dependent on the used stimuli. The QuantiFERON-CMV assay targets CD8 + T Go 6976 by using peptides whereas the T-Track-CMV targets CD4+ and CD8 + T cells by stimulation with two viral proteins [18]. In our study, a specificity of 100% wa
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In addition to PGC transcriptional co repressors such
2020-08-20
In addition to PGC1α, transcriptional co-repressors such as NCOR and RIP140 participate in oxidative muscle remodeling induced by exercise, whereby reductions in their expression and the resulting de-repression of downstream TFs activates oxidative gene expression (Seth et al., 2007, Yamamoto et al.
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E protein was studied using specific anti E
2020-08-20
E1 protein was studied using specific anti-E1 s7694 in wild type cells and in transfected cells overexpressing the enzyme. The results revealed several unusual characteristics, which are unique to this ubiquitin-activating enzyme. The E1 was translated as a full-length protein and then quickly prot
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A An optimized BEI SEIscore based on the
2020-08-19
(A) An “optimized BEI-SEIscore” based on the trends, emerged from the statistical analysis of several optimization studies in pharmaceutical industry (Abad-Zapatero, 2007, Abad-Zapatero and Blasi, 2011). This type of optimized scores has been used in many previous studies. Ki=1nM at 300° K, compare
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A An optimized BEI SEIscore based on the
2020-08-19
(A) An “optimized BEI-SEIscore” based on the trends, emerged from the statistical analysis of several optimization studies in pharmaceutical industry (Abad-Zapatero, 2007, Abad-Zapatero and Blasi, 2011). This type of optimized scores has been used in many previous studies. Ki=1nM at 300° K, compare
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