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The objective of this work was to
2018-10-22
The objective of this work was to investigate if a synergistic coculture system of MSCs with endothelial sr9009 could affect the number and state of differentiation of harvested MSCs. In contrast to other papers, in this cell-cell communication topic, the investigation was carried out for a longer
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As expected PA could decrease the viability of NSCs
2018-10-22
As expected, PA could decrease the viability of NSCs in a dose- and time-dependent manner. In addition to its adverse effects on cell morphology, our speculation that PA might also be involved in apoptosis of NSCs was confirmed by TUNEL assay. Very interestingly, the effects of PA on apoptosis of NS
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The goals of the current study were to investigate
2018-10-22
The goals of the current study were to investigate the changes in anal sphincter pressure after IV or IM injection of MSCs in our previously established animal models. Our models (Salcedo et al., 2010; Zutshi et al., 2009a) allow measurement of anal sphincter pressure and EMG in vivo in a repeated,
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To identify a molecular marker that can
2018-10-22
To identify a molecular marker that can serve as an indicator of the differentiaion potential of the cell lines tested, we analyzed the p2x receptors levels of a group of genes in hESCs that include pluripotent transcription factors, modulators of various signaling pathways known to be involved in
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In order to enhance osteochondral commitment
2018-10-22
In order to enhance osteochondral commitment, we performed chondrogenic pre-induction of ATSC/β-TCP constructs. Ectopic bone formation was installed in more than 75% of samples, suggesting that a sufficient degree of determination was reached to obtain about as much new bone as with non-induced BMSC
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This is in accord with previous reports that
2018-10-22
This is in accord with previous reports, that astrocytes influence both proliferation and differentiation of embryonic and adult neural stem cells, and specifically promote neurogenic fate of neural stem cells (Roy et al., 2006; Nakayama et al., 2003; Song et al., 2002). Factors that affect neural s
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br Community demand and expectation Unfortunately
2018-10-20
Community demand and expectation Unfortunately, autologous stem cell therapies continue to be presented and viewed under a commercialistic social lens which remains insensitive to the medical professional norms just described. This makes it all too easy for clinicians to offer these treatments as
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br Methods br Results br Conclusion br
2018-10-20
Methods Results Conclusion Author contributions The following are the supplementary data related to this article. Acknowledgments Stemformatics is funded by a grant from the Australian Research Council Special Initiative in Stem Cell Science to CAW through Stem Cells Australia. Stemf
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Paradoxically we found that mutant K
2018-10-20
Paradoxically, we found that mutant K-Ras increases proliferation of quiescent intestinal stem casin (Fig. 2), but also increases repair following damage (Fig. 3). This phenomenon is similar to the role that oncogenic N-Ras plays in hematopoietic stem cells (HSCs) (Li et al., 2013). In this context,
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br Materials and methods br
2018-10-20
Materials and methods Results Discussion We have previously shown that cellular phenotype is an important determinant of the malignant potential in human neuroblastoma dilution factor calculator and tumors (Walton et al., 2004). In particular, in those experiments, we identified a stem cell
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Subsequently both FGF b and FGF treatments were found
2018-10-20
Subsequently, both FGF8b and FGF10 treatments were found to initiate Lim3+ adenohypophysis progenitor induction. The Pitx1+ oral ectoderm and Rax+ hypothalamus of SQ 22536 in BMP4-treated culture were similar to the oral ectoderm and hypothalamus seen in the TTF-1 KO mouse [2]. In such an embryo, B
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Human fibroblasts were reprogrammed from an
2018-10-20
Human fibroblasts were reprogrammed from an individual into nociceptor neurons without creation of iPSCs and the neurons exhibited sensitization to the chemotherapeutic drug oxaliplatin, modeling the inherent mechanisms underlying painful CIPN (Wainger et al., 2015); however, the advantage to creati
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br Materials and methods br Results br Discussion The
2018-10-20
Materials and methods Results Discussion The present study characterises the early effects and safety profile of hMSC therapy in a large animal model of ALI. After 4h of treatment, we have demonstrated that hMSC reduced tissue NF-κB, an important transcription factor that mediates I-BET-762
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br Experimental Procedures A detailed version
2018-10-20
Experimental Procedures A detailed version of our Experimental Procedures can be found in the Supplemental Experimental Procedures. All animal experiments were approved by the Danish Council for Supervision with Experimental Animals (#2011/561-1966). AR was performed at P1 as previously described
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In summary these perivascular derivatives demonstrate
2018-10-20
In summary, these perivascular derivatives demonstrate an important building block toward not only reconstructing physiologically relevant vasculature but also the study of developmental processes and diseases implicating these cell types. Important elements of our system are the several noted discr
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